Pii: S0041-0101(00)00194-x
نویسندگان
چکیده
The present report describes the use of ELISA with cDNA expression libraries in the identification of immunogenic proteins. The methodology described was applied using libraries constructed with mRNA isolated from Tityus serrulatus and Tityus bahiensis venom glands. In addition we describe for the first time the sequence of a neurotoxin from Tityus bahiensis venom gland named TbTx5 whose amino acid sequencing showed 93% similarity with the Tityus bahiensis TbTx IV-5 neurotoxin. The methodology described can be used for the generation of an immunogenic bank in order to contribute to genome and proteome projects. q 2000 Elsevier Science Ltd. All rights reserved.
منابع مشابه
Pii: S0041-0101(00)00154-9
This work presents a study of the importance of natural products, especially those derived from higher plants, in terms of drug development. It describes the main strategies for obtaining drugs from natural sources, fields of knowledge involved, difficulties and perspectives. It also includes a brief discussion of the specific situation in Brazil regarding the use of, trade in, and research int...
متن کاملPii: S0041-0101(00)00224-5
A previously undescribed cyclic heptapeptide hepatotoxin was isolated from a cyanobacteria waterbloom collected in Pakowki Lake, Alberta, Canada (49820 0N and 110855 0W). The compound was characterized by amino acid analysis, ESIMS/CID/MS, H and C NMR, and UV spectroscopy. Structure of the new microcystin was assigned as [d-Leu]microcystin-LR (1). The amino acid composition is the same as micro...
متن کاملPii: S0041-0101(00)00237-3
We have isolated and characterized a novel hemolytic protein from the venom of the Hawaiian box jelly®sh (Carybdea alata). Hemolysis of sheep red blood cells was used to quantitate hemolytic potency of crude venom extracted from isolated nematocysts and venom after fractionation and puri®cation procedures. Hemolytic activity of crude venom was reduced or lost after exposure to the proteolytic e...
متن کاملPii: S0955-0674(00)00194-0
Hypoxia-inducible factor 1 (HIF-1) is an oxygen-regulated transcriptional activator that plays essential roles in mammalian development, physiology and disease pathogenesis. The HIF-1α subunit is subjected to oxygen-dependent ubiquitination and proteasomal degradation that is mediated by the von Hippel-Lindau protein. Interaction of HIF-1α transactivation domains with coactivators is induced by...
متن کاملPii: S0140-3664(00)00194-8
Ef®cient resource utilisation in future cellular systems is related to the control of the signalling load imposed by the location update and paging operations. Important means for controlling this load is the apropero planning of location areas. In this paper we de®ne and solve a version of the location area planning problem. Starting from a formal de®nition and an optimal formulation of the pr...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2000